Donald A. Primerano, PhD

Donald Primerano, Ph.D.Professor; Microbiology Section Head; and
Co-Director, Genomics and Bioinformatics Core Facility
Department: Biochemistry and Microbiology
Office: BBSC 336-F | Laboratories: BBSC 317, 318 and 326
Phone: (304) 696-7338 | Fax: (304) 696-7207

Research Interests

My primary research interests are in the discovery of cancer and cardiovascular susceptibility genes using re-sequencing, expression profiling and bioinformatic approaches. I currently serve as the Co-Director of the Genomics and Bioinformatics Core Facility (GABC), and as a member of WV-INBRE Administrative Core and WV Cancer Genomics Steering Committee. As Co-Director of the GABC, I have experience in (1) developing sequencing strategies and service relationships between the core and principal investigators needing genomic analyses, (2) in providing overall direction to a core with evolving technologies and institutional responsibilities, and (3) in assisting individual investigators in designing genomic experiments. During the period from 1999 to the present, the Genomics Core has successfully supported the goals of the several funded programs: WVBRIN, WV-INBRE Phase I, WV-INBRE phase II, COBRE Transcription Factors and Cancer, and the WV Cancer Genomics Network. Under my direction, the GABC has launched next generation sequencing as a state-wide service and completed several NGS projects including RNA-Seq, whole exome sequencing, whole genome sequencing, and microbiome studies. My ongoing projects include studies directed at discovering variants associated with susceptibility to infectious disease, diabetes and obesity.

Recent Publications

  1. Lauren L. Richards-Waugh*, Donald A. Primerano, Yulia Dementieva, James C. Kraner, and Gary O. Rankin Fatal Methadone Toxicity: Potential Role of CYP3A4 Genetic Polymorphism (JAT-14-1598) accepted for publication in Journal of Analytical Toxicology, (2014)
  2. Fahrmann JF, Ballester OF, Ballester G, Witte TR, Salazar AJ, Kordusky B, Cowen KG, Ion G, Primerano DA, Boskovic G, Denvir J, Hardman WE. Inhibition of nuclear factor kappa B activation in early-stage chronic lymphocytic leukemia by omega-3 fatty acids. Cancer Invest. 31: 24-38 (2013) PMID: 23193970
  3. D. Primerano, G. Boskovic, J. Fan, J. H. Kim and J.Denvir. Potential Susceptibility Variants for Obesity and Type 2 Diabetes in the TALLYHO Mouse. J Biomolecular Tech. 23(Suppl): S41. (2012) PMCID: PMC3630602
  4. Cockburn AF, Dehlin JM, Ngan T, Crout R, Boskovic G, Denvir J, Primerano D, Plassman BL, Wu B, Cuff CF. High throughput DNA sequencing to detect differences in the subgingival plaque microbiome in elderly subjects with and without dementia. Investigative Genetics 3:19 (2012) PMCID: PMC3488532, PMID: 22998923 [PubMed]
  5. OM Ballester, G Ballester, TR Witte, AJ Salazar, G Ion, DA Primerano, G Boskovic, J Denvir, W. Elaine Hardman. Inhibition of Nuclear Factor Kappa B activation in Early Stage Chronic Lymphocytic Leukemia by Omega 3 Fatty Acids. JF Fahrmann. Cancer Investigation 31: 24-38 (2013). PMID: 23193970
  6. Stephanie L. Rellick, Heather O’Leary, Debbie Piktel, Cheryl Walton, James E. Fortney, Stephen M. Akers, Karen H. Martin, James Denvir, Goran Boskovic, Donald A. Primerano, Jeffrey Vos, Nathanael Bailey, Marieta Gencheva, and Laura F. Gibson. Bone Marrow Osteoblast Damage by Chemotherapeutic Agents. PLoS One. 7:e30758 (2012) PMCID: PMC3281873
  7. The Appalachian Cardiovascular Research Network (Yulia Dementieva*, J. Denvir, L. Wei, DA Primerano, T. L. Green, P. Wehner, M. R. Flood, D. Calica, B. Freeman, M. Huff, S. Dodson, C. Hill, A. Francis, K. McIntyre, R. Kreisberg, S. Warren, H. Blackwood, M. Davis, H-M Lee.) Identification of genes contributing to cardiovascular disease in overweight and obese individuals from West Virginia. WV Medical Journal 108: 23-31 (2012)

Laboratory Personnel

Jun Fan, Ph.D. – Next Generation Sequencing Manager