Donald A. Primerano, PhD

Donald Primerano, Ph.D.Professor, and Interim Chair of Biochemistry and Microbiology
Co-Director, Genomics and Bioinformatics Core Facility
Department: Biochemistry and Microbiology
Office: BBSC 336-F | Laboratories: BBSC 317, 318 and 326
Phone: (304) 696-7338 | Fax: (304) 696-7207

Research Interests

My primary research interests are in the discovery of cancer and cardiovascular susceptibility genes using re-sequencing, expression profiling and bioinformatic approaches. I currently serve as the Co-Director of the Genomics and Bioinformatics Core Facility (GABC), and as a member of WV-INBRE Administrative Core and WV Cancer Genomics Steering Committee. As Co-Director of the GABC, I have experience in (1) developing sequencing strategies and service relationships between the core and principal investigators needing genomic analyses, (2) in providing overall direction to a core with evolving technologies and institutional responsibilities, and (3) in assisting individual investigators in designing genomic experiments. During the period from 1999 to the present, the Genomics Core has successfully supported the goals of the several funded programs: WVBRIN, WV-INBRE Phase I, WV-INBRE phase II, COBRE Transcription Factors and Cancer, and the WV Cancer Genomics Network. The GABC currently provides whole genome sequencing, whole exome sequencing, transcriptome expression profiling (RNA-Seq) and microbiome studies as well as bioinformatic analyses that correspond to these applications. My ongoing projects include studies directed at discovering variants associated with susceptibility to diabetes and obesity.

Recent Publications

  1. Justin K. Tomblin, Subha Arthur, Donald A. Primerano, Ateeq R. Chaudhry, Jun Fan, James Denvir and Travis B Salisbury. Aryl hydrocarbon receptor (AHR) regulation of L-Type Amino Acid Transporter 1 (LAT-1) expression in MCF-7 and MDA-MB-231 breast cancer cells. Accepted for publication.
  2. Denvir J, Neitch S, Fan J, Niles RM, Boskovic G, Schreurs BG, Primerano DA, and Alkon DL. Identification of the PS1 Thr147Ile Variant in a Family with Very Early Onset Dementia and Expressive Aphasia. J Alzheimers Dis. 2015 May 30; 46(2): 483–490. doi: PMCID: PMC4583332
  3. Miranda B. Carper, James Denvir, Goran Boskovic, Donald A. Primerano, and Pier Paolo Claudio. RGS16, a novel p53 and pRb cross-talk candidate inhibits migration and invasion of pancreatic cancer cells. Genes and Cancer 5:420-435 (2014) [PMID: 25568667 [PubMed] PMCID: PMC4279439]
  4. Travis B Salisbury, Justin K. Tomblin, Donald A. Primerano, Goran Boskovic, Jun Fan, Jackie Fletcher, Nalini Santanam, Estil Hurn, Gary Z. Morris, and James Denvir. Endogenous aryl hydrocarbon receptor promotes basal and inducible expression of tumor necrosis factor target genes in MCF-7 cancer cells. Biochem Pharmacol 91:390-9 (2014). PMID: 24971714 PMCID: PMC4157967
  5. Lauren L. Richards-Waugh*, Donald A. Primerano, Yulia Dementieva, James C. Kraner, and Gary O. Rankin Fatal Methadone Toxicity: Potential Role of CYP3A4 Genetic Polymorphism (JAT-14-1598) accepted for publication in Journal of Analytical Toxicology, (2014)
  6. Fahrmann JF, Ballester OF, Ballester G, Witte TR, Salazar AJ, Kordusky B, Cowen KG, Ion G, Primerano DA, Boskovic G, Denvir J, Hardman WE. Inhibition of nuclear factor kappa B activation in early-stage chronic lymphocytic leukemia by omega-3 fatty acids. Cancer Invest. 31: 24-38 (2013) PMID: 23193970
  7. D. Primerano, G. Boskovic, J. Fan, J. H. Kim and J.Denvir. Potential Susceptibility Variants for Obesity and Type 2 Diabetes in the TALLYHO Mouse. J Biomolecular Tech. 23(Suppl): S41. (2012) PMCID: PMC3630602
  8. Cockburn AF, Dehlin JM, Ngan T, Crout R, Boskovic G, Denvir J, Primerano D, Plassman BL, Wu B, Cuff CF. High throughput DNA sequencing to detect differences in the subgingival plaque microbiome in elderly subjects with and without dementia. Investigative Genetics 3:19 (2012) PMCID: PMC3488532, PMID: 22998923 [PubMed]
  9. OM Ballester, G Ballester, TR Witte, AJ Salazar, G Ion, DA Primerano, G Boskovic, J Denvir, W. Elaine Hardman. Inhibition of Nuclear Factor Kappa B activation in Early Stage Chronic Lymphocytic Leukemia by Omega 3 Fatty Acids. JF Fahrmann. Cancer Investigation 31: 24-38 (2013). PMID: 23193970
  10. Stephanie L. Rellick, Heather O’Leary, Debbie Piktel, Cheryl Walton, James E. Fortney, Stephen M. Akers, Karen H. Martin, James Denvir, Goran Boskovic, Donald A. Primerano, Jeffrey Vos, Nathanael Bailey, Marieta Gencheva, and Laura F. Gibson. Bone Marrow Osteoblast Damage by Chemotherapeutic Agents. PLoS One. 7:e30758 (2012) PMCID: PMC3281873
  11. The Appalachian Cardiovascular Research Network (Yulia Dementieva*, J. Denvir, L. Wei, DA Primerano, T. L. Green, P. Wehner, M. R. Flood, D. Calica, B. Freeman, M. Huff, S. Dodson, C. Hill, A. Francis, K. McIntyre, R. Kreisberg, S. Warren, H. Blackwood, M. Davis, H-M Lee.) Identification of genes contributing to cardiovascular disease in overweight and obese individuals from West Virginia. WV Medical Journal 108: 23-31 (2012)

Laboratory Personnel

Jun Fan, PhD – Next Generation Sequencing (NGS) Manager
Swanthana Rekullapally MS: NGS Data Analyst