Department: Biological Sciences; Biochemistry and Molecular Biology
Office: BBSC 241-P | Laboratory: BBSC 243
Phone: (304) 696-3965 | Fax: (304) 696-7136
My laboratory is working on the role of sulforaphane (SFN), an isothiocynate compound present in cruciferous vegetables, in epigenetic modifications in the context of prostate cancer. The role of DNA methylation, histone post-translational modifications (PTM), and chromatin folding are investigated. Our initial focus was on two genes affected by SFN treatment, the tumor suppressor p21 (up-regulated) and the human Telomerase Reverse-Transcriptase (hTERT, down-regulated). In collaboration with Dr. Gordon Hager (NIH), Emily Ho (Oregon State University), and Fahd Almulla (Kuwait University), we are specifically looking at the genome-wide changes in distribution of histone PTMs and recruitment of Histone De-Acetylases (HDAC) in response to SFN treatment. A second project related to diet (in collaboration with Dr. Elaine Hardman), investigates the effect of omega 3 fatty acids on breast cancer in mice. We are focusing on epigenetic mechanism of imprinting related to diet. Lastly, my laboratory has recently identified a novel mechanism of gene regulation implicating several chromatin remodeling complexes and leading to cell differentiation (using a salivary gland-differentiation deficient mouse model). This novel regulation mechanism may also involve differential splicing.
Breaking the cycle: the role of omega-3 polyunsaturated fatty acids in inflammation-driven cancers. Patterson W.L. and Georgel P.T. Biochemistry and Cell Biology (in press)
The epigenetic potential of dietary polyphenols in prostate cancer. Abbas, A., Patterson, W., and Georgel P.T. Biochemistry and Cell Biology Vol. 91. Pp361-368 (2013)
MeCP2, Structure and Function. Adkins, N.L. and Georgel, P.T. Biochemistry and Cell Biology Vol. 89. Pp1-11 (2011)
MeCP2 binds to nucleosomes free (linker DNA) regions and to H3K9/H3K27 methylated nucleosomes in the brain. Anita A. Thambirajah, Marlee K. Ng, Lindsay J. Frehlick, Andra Li, Jason J. Serpa, Evgeniy V. Petrotchenko, Begonia Silva-Moreno, Kristal K. Missiaen, Christoph H. Borchers, J. Adam Hall, Ryan Mackie, Frank Lutz, Brent E. Gowen, Michael Hendzel, Philippe T. Georgel and Juan Ausio. Nucleic Acids Research (2011)
Silencing and Re-expression of Retinoic Acid Receptor Beta2 in Human Melanoma. Fan J, Eastham, L., Varney, M., Hall, A., Adkins, N.L., Sollars, V., Georgel, P., Niles, R.M. Pigment Cell Melanoma Res. Vol. 23. Pp 419-429 (2010). [Epub 2010 Mar29]
Chromatin Stability at Low Concentration Depends on Histone Octamer Saturation Levels. Hagerman, T., Fu, Q., Molinié, B., Lindsay, S., Georgel, P.T. Biophysical Journal, Vol. 96. Pp 1944-1951, (2009)
Role of nucleic acid binding in Sir3-dependent interactions with chromatin fibers. Adkins, N.L., McBryant, S., Johnson, C. N., Leidy, J.M., Woodcock, C.L., Robert C.H., Hansen, J.C., and Georgel, P.T. Biochemistry, Vol. 48. Pp 276-288, (2009).
The use of Quantitative Agarose Gel Electrophoresis for rapid analysis of the integrity of protein-DNA complexes. Adkins, N.L., Hall, J.A. and Georgel, P.T. Journal of Biochemical and Biophysical Methods. Vol. 70. Pp721-726 (2007).
Characterization of chromatin samples in the presence of Drosophila embryo extract by Quantitative Agarose Gel Electrophoresis. Adkins, N.L., Johnson, C.N. and Georgel, P.T. Journal of Biochemical and Biophysical Methods. Vol. 67. Pp 141-150 (2006).
HDAC1 acetylation is linked to progressive modulation of steroid receptor induced gene transcription. Qiu,Y., Zhao,Y., Becker,M., John,S., Parekh,B.S., Huang,S., Hendarwanto,A., Martinez,E.D., Chen,Y., Lu,H., Adkins,N.L., Stavreva,D.A., Wiench,M., Georgel,P.T., Schiltz,R.L., and Hager,G.L. Molecular Cell.Vol. 22 (5). Pp 669-679 (2006).
Diane Dawley – MD/PhD student
Daniel Crow – Capstone student