Vice Dean, Chairman, Department of Clinical and Translational Sciences | Chief Medical Informatics Officer
Joan C. Edwards School of Medicine
Office: McKown Translational Genomic Research Institute
Lab: Byrd Biotechnology Science Center 312
Phone: 304-691-1841 | Fax: 304-691-1840
The Clinical and Translational laboratory research broadly focuses on understanding the function and regulation of intestinal electrolyte and nutrient co-transporters and how they might be regulated during chronic conditions such as intestinal inflammation as seen in Inflammatory Bowel Disease (IBD) and colon cancer.
IBD is manifested with key symptoms such as diarrhea, malnutrition and weight loss. In the majority of IBD cases, patients are unable to properly assimilate chemicals, water, and nutrients that are critical for daily life leading to symptoms such as malnutrition, weight loss, and diarrhea, which in most cases prove fatal. Steroids are the only treatment option currently available for patients with IBD. Though steroids improve or put the disease in remission (a period without symptoms), they have been reported to have numerous side effects causing additional discomfort to the patients. Therefore, it becomes important to identify the bodily immune substances and molecular mechanisms that might interfere with the absorption of specific nutrients and electrolytes in the intestine. The biological molecules thus identified can be used as targets for drug development and treatment thus replacing steroids for IBD treatment.
As a first and significant step of our research goal, we have successfully established the molecular identity of all the essential nutrient and electrolyte transporters (sodium, glucose, amino acids etc.). These form the primary absorptive mechanism in the brush border membrane of the intestinal cells and also how they are regulated during intestinal inflammation. We are currently working on identifying and establishing the potential biological drug treatment targets that might specifically regulate these intestinal transporters during chronic intestinal inflammation.
For a full listing of publications, please access PubMed.
Regulation of sodium glucose co-transporter SGLT1 through altered glycosylation in the intestinal epithelial cells. Arthur S, Coon S, Kekuda R, Sundaram U. Biochim Biophys Acta. 2014 May;1838(5):1208-14. PMID:24412219
Monocarboxylate 4 mediated butyrate transport in a rat intestinal epithelial cell line. Kekuda R, Manoharan P, Baseler W, Sundaram U. Dig Dis Sci. 2013 Mar;58(3):660-7. PMID: 23344966
Prostaglandins, not the leukotrienes, regulate Cl(-)/HCO(3)(-) exchange (DRA, SLC26A3) in villus cells in the chronically inflamed rabbit ileum. Manoharan P, Coon S, Baseler W, Sundaram S, Kekuda R, Sundaram U. Biochim Biophys Acta. 2013 Feb;1828(2):179-86. PMID: 22963933
Regulation of sodium-glutamine cotransport in villus and crypt cells by glucocorticoids during chronic enteritis. Arthur S, Saha P, Sundaram S, Kekuda R, Sundaram U. Inflamm Bowel Dis. 2012 Nov;18(11):2149-57. PMID: 22508450
Na-glutamine co-transporters B(0)AT1 in villus and SN2 in crypts are differentially altered in chronically inflamed rabbit intestine. Saha P, Arthur S, Kekuda R, Sundaram U. Biochim Biophys Acta. 2012 Mar;1818(3):434-42. PMID: 22100603
Utility of upper endoscopy and colonoscopy in evaluating gastrointestinal luminal wall thickening found on computed tomography. Karim MS, Miranda MC, Shamma’a JM, Goebel SU, Sundaram U. W V Med J. 2010 Nov-Dec;106(7):16-9. PMID: 21932485
Correlation between MRCP and ERCP findings at a tertiary care hospital. Rahman R, Ju J, Shamma’s J, Goebel S, Sundaram U. W V Med J. 2010 Jul-Aug;106(5):14-9. PMID: 21739880
Reciprocal regulation of the primary sodium absorptive pathways in rat intestinal epithelial cells. Coon S, Kekuda R, Saha P, Sundaram U. Am J Physiol Cell Physiol. 2011 Mar;300(3):C496-505. PMID: 21148403
Endoscopic approach to capsule endoscope retention. Roorda AK, Kupec JT, Ostrinsky Y, Shamma’a JM, Goebel SU, Sundaram U. Expert Rev Gastroenterol Hepatol. 2010 Dec;4(6):713-21. PMID: 21108591
Glucocorticoids differentially regulate Na-bile acid cotransport in normal and chronically inflamed rabbit ileal villus cells. Coon S, Kekuda R, Saha P, Sundaram U. Am J Physiol Gastrointest Liver Physiol. 2010 May;298(5):G675-82. PMID: 20075140
Bupropion and naltrexone: a review of their use individually and in combination for the treatment of obesity. Plodkowski RA, Nguyen Q, Sundaram U, Nguyen L, Chau DL, St Jeor S. Expert Opin Pharmacother. 2009 Apr;10(6):1069-81. PMID: 19364254
The phenotype of persons having mosaicism for trisomy 21/Down syndrome reflects the percentage of trisomic cells present in different tissues. Papavassiliou P, York TP, Gursoy N, Hill G, Nicely LV, Sundaram U, McClain A, Aggen SH, Eaves L, Riley B, Jackson-Cook C.Am J Med Genet A. 2009 Feb 15;149A(4):573-83. PMID:19291777
Mechanism of leukotriene D4 inhibition of Na-alanine cotransport in intestinal epithelial cells. Talukder JR, Kekuda R, Saha P, Sundaram U. Am J Physiol Gastrointest Liver Physiol. 2008 Jul;295(1):G1-G6. PMID: 18436626
Identification and characterization of rabbit small intestinal villus cell brush border membrane Na-glutamine cotransporter. Talukder JR, Kekuda R, Saha P, Arthur S, Sundaram U.Am J Physiol Gastrointest Liver Physiol. 2008 Jul;295(1):G7-G15. PMID: 18436625
Functional characterization, localization, and molecular identification of rabbit intestinal N-amino acid transporter. Talukder JR, Kekuda R, Saha P, Prasad PD, Ganapathy V, Sundaram U. Am J Physiol Gastrointest Liver Physiol. 2008 Jun;294(6):G1301-10. PMID: 18388187
Role of Sp1 and HNF1 transcription factors in SGLT1 regulation during chronic intestinal inflammation. Kekuda R, Saha P, Sundaram U. Am J Physiol Gastrointest Liver Physiol. 2008 Jun;294(6):G1354-61. PMID:18339704
Constitutive nitric oxide differentially regulates Na-H and Na-glucose cotransport in intestinal epithelial cells. Coon S, Kekuda R, Saha P, Talukder JR, Sundaram U. Am J Physiol Gastrointest Liver Physiol. 2008 Jun;294(6):G1369-75. PMID: 18325982
Primary amenorrhea and absent uterus in the 22q11.2 deletion syndrome. Sundaram UT, McDonald-McGinn DM, Huff D, Emanuel BS, Zackai EH, Driscoll DA, Bodurtha J. Am J Med Genet A. 2007 Sep 1;143A(17):2016-8. PMID: 17676598
Mechanism of regulation of rabbit intestinal villus cell brush border membrane Na/H exchange by nitric oxide. Coon S, Shao G, Wisel S, Vulaupalli R, Sundaram U. Am J Physiol Gastrointest Liver Physiol. 2007 Feb;292(2):G475-81. PMID: 17290013
Neutral Na-amino acid cotransport is differentially regulated by glucocorticoids in the normal and chronically inflamed rabbit small intestine. Sundaram U, Wisel S, Coon S. Am J Physiol Gastrointest Liver Physiol. 2007 Feb;292(2):G467-74. PMID: 17290012
Subha Arthur, PhD – Assistant Professor
Soudamani Singh, PhD – Postdoctoral Fellow
Balasubramanian Palaniappan, PhD – Postdoctoral Fellow