Genomics Core Facility

The Genomics Core Facility includes the Microarray Core Facility and the Automated DNA Sequencing Service.

Microarray Core Facility

General

We provide microarray-based gene expression profiling based on an “all Agilent” workflow. This service includes RNA labeling, hybridization, image acquisition, and data analysis. Prior to the initiation of any microarray experimentation (e.g. expression profiling), investigators must discuss experimental goals, design, expectations, RNA requirements, time of completion, and overall cost of the proposed experiment with microarray core personnel. Investigators will be provided with proven RNA extraction protocols and instructed to provide sufficient quantities of total RNA (currently 100 ng). Investigators outside of Marshall University are required to ship total RNA as a precipitate on dry ice by next day Fedex.

Automated DNA Sequencing Service

We provide dye terminator sequencing for plasmid and PCR templates using the ABI 3130 Genetic Analyzer with the capacity to sequence 96 templates per day. In dye terminator sequencing, DNA fragments are labeled by incorporation of a dye labeled dideoxynucleotide. Investigators must provide unlabeled primers and purified template DNA. Our read lengths average 800 bp for column-purified plasmid DNA or PCR products. Please complete our online sequencing request form prior to mailing your template DNA and primer.

To learn more about the this facility, refer to the Genomics Core Facility website.