Document Details

Document Type:   Thesis
Title:   Characterization and Differentiation of Peripheral Blood Derived Multipotent Adult Progenitor Cells Multipotent Adult Progenitor Cells
Author:   Hari Satya Shankar Addagarla
College:   Science
Degree Program:   Biological Sciences, M.S.
Degree:   Master of Science
Committee Director:   Dr. Elmer M. Price
Document Availability:   Document available for World-Wide access.
Date of Defense:   07/23/2009

Stem cells are populations of undifferentiated cells found in most tissues. Our lab developed a novel cell line from the peripheral blood of adult transgenic green fluorescent protein swine and designated them as Peripheral Blood Derived Multipotent Adult Progenitor Cells (PBD-MAPCs). In this study we characterized the expression profiles of PBD-MAPCs before and after neural differentiation and investigated the potential of PBD-MAPCs to differentiate into myocardial or neural lineages. We examined the potential of various cytokines and co-culture methods to differentiate PBD-MAPCs into cardiomyocytes. To study the expression profiles, we probed the expression of stem cell and neural markers in PBD-MAPCs before and after neural differentiation. Finally, we performed 3 dimensional cell cultures on PBD-MAPCs using various biomaterials as a preliminary study to develop a bridging transplant for spinal cord injuries. Our data suggest that PBD-MAPCs can be a promising source for research in cellular therapeutics for spinal cord injury.  

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