Document Details

Document Type:   Thesis
Title:   Lean and Obese Zucker Rats Exhibit Different Patterns of p70S6kinase Regulation in the Tibialis Anterior Muscle in Response to High Force Muscle Contraction
Author:   Anjaiah Katta
College:   Science
Degree Program:   Biological Sciences, M.S.
Degree:   Master of Science
Committee Director:   Dr. Eric R Blough
Document Availability:   Document available for World-Wide access.
Date of Defense:   08/02/2007

Increased muscle loading results in phosphorylation of the 70 kDa ribosomal S6 kinase (p70s6k) signaling pathway and this event strongly correlates with degree of muscle adaptation following resistance exercise. Here, we compared the basal and contraction-induced phosphorylation of p70s6k, Akt and mTOR in tibialis anterior muscles of lean and obese Zucker rats.Immunoblotting demonstrated differences in level of basal p70S6k phosphorylation (Thr 389) in the normal and diabetic TA. HFES had an increase in p70S6k (Thr389) phosphorylation at 0-, 1- and 3-hr in lean TA and only at 3-hr in obese TA. mTOR (Ser 2448) Phosphorylation was elevated in lean TA immediately after HFES and remains unaltered in obese TA. HFES increased activity of both Akt (Thr 308) and Akt (Ser 473) in lean TA.These results suggest that diabetes is associated with alterations in the muscle content and ability to activate p70s6k signaling following an acute bout of exercise. 

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