Document Details

Document Type:   Thesis
Title:   Impaired Overload-Induced Hypertrophy in Obese Zucker Rat Slow-Twitch Skeletal Muscle skeletal muscle
Author:   Anil Kumar Gutta
College:   Science
Degree Program:   Biological Sciences, M.S.
Degree:   Master of Science
Committee Director:   Dr.Eric R Blough
Document Availability:   Document available for World-Wide access.
Date of Defense:   07-30-2008

How insulin resistant muscle responds to mechanical overload (MOV) is not well understood. Using immunohistochemical analysis and immunoblotting, we investigated whether Lean Zucker (LZ) and insulin resistant Obese Zucker rats (OZ) respond in a similar fashion to MOV. Five young adult (2 months old) male LZ and OZ rats were subjected to a surgical ablation of the gastrocnemius muscle and overloaded for 8 weeks. MOV-induced increases in soleus muscle mass and average fiber cross-sectional area were attenuated in OZ compared to LZ animals. This reduction in OZ muscle adaptation was associated with decreased activity / phosphorylation levels of STAT3, β-catenin and calcineurin. These data suggest that insulin resistance may decrease the ability of skeletal muscle to hypertrophy and that this impairment may be due to alterations in the ability of insulin-resistant muscle to activate STAT3, β-catenin and calcineurin signaling.  

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