Research Day 2011 – Brandy Maydon – Validation of the Applied Biosystems AmpFlSTR® Yfiler™ PCR Amplification Kit

Validation of the Applied Biosystems AmpFlSTR® Yfiler™ PCR Amplification Kit

Biography:

Brandy Jean Maydon grew up in La Feria, Texas. After graduating from high school, she attended Texas A&M University-Corpus Christi where she earned a bachelor’s degree in Biomedical Forensic Science with an emphasis in Forensic Science in 2009. She is currently attending Marshall University, where she is working towards a degree in Forensic Science with emphasis’ in forensic DNA analysis and Digital Forensics. This summer, Brandy worked in the Miami Dade Crime Lab in Miami, Florida and today she will present on that work titled: Validation of the Applied Biosystems AmpFlSTR® Yfiler™ PCR Amplification Kit.

Abstract:

The Yfiler kit is used to produce male specific profiles by utilizing loci from the Y chromosome. Seven studies observing sensitivity, precision, inhibition, mixtures, nonprobative casework, nonhuman DNA and family samples were performed to determine the kits optimal parameters for forensic casework samples. The kit was shown to be human male specific with no interference occurring due to the presence of female DNA, and identical profiles were produced between paternally linked males. Yfiler showed a sensitivity range between 0.15-1.5 ng/µl and high precision. Successful amplification of DNA was seen in casework samples but inhibition can occur in the presence of rhodamine and NaCl. Further studies manipulating inhibition concentrations and sensitivity to touch DNA compared to Identifiler deserve further inquiry.

Poster:

Validation of the Applied Biosystems AmpFlSTR® Yfiler™ PCR Amplification Kit

References:

  1. Koilff, Peter de. “Son, Give Up Your Gun: Presenting Y-STR Results in Court”. Profiles in DNA. September 2003.
  2.  Julio J. Mulero,1 Ph.D.; Chien Wei Chang,1 Ph.D.; Lisa M. Calandro,1 M.P.H.; Robert L. Green,1 B.A.;Yixin Li,1 Ph.D.; Cassie L. Johnson, M.S.; and Lori K. Hennessy,1 Ph.D. “Development and Validation of the AmpF‘STRsYfilerTM PCR Amplification Kit: A Male Specific,Single Amplification 17 Y-STR MultiplexSystem.” J Forensic Sci, January 2006, Vol. 51, No. 1
  3.  Kimberly A. Sturk, Michael D. Coble, Suzanne M. Barritt, Jodi A. Irwin “Evaluation of Modified Yfiler Amplification Strategy for Compromised Samples.” Croat Med J. 2009; 50: 228-38
  4.  Kathleen A. Mayntz-Press, M.S. and Jack Ballantyne,Ph.D. “Performance Characteristics of Commercial Y-STR Multiplex Systems*” Forensic Sci, September 2007, Vol. 52, No. 5