Biorad/Zeiss MRC 1024 with TLD (Transmitted Light Detector) on Inverted Nikon Diaphot 300 with DIC Optics and 6.2 Megapixel Nikon SLR with Mecury Lamp Epifluorescence.
The confocal microscope is a form of microscopy that permits examination of living cells without leading to their demise. Using an argon laser light source, confocal microscopy produces sharp fluorescent images because light from only one focal plane is used to produce the image. Light from other "out of focus" planes is blocked by the detection pinhole, part of the optical system of the microscope (see an animation of how the confocal microscope works). The emitted/reflected light passing through the detector pinhole is distributed into three different wavelength channels, transformed into electrical signals by photomultiplier tubes and displayed on a computer monitor screen.
There are a number of benefits associated with the use of the confocal microscope. These include:
These tools and others are utilized to aid in characterizing and understanding nanomachines, molecular systems and cell biology through research. To discuss use of the system and/or other imaging systems of interest, please contact Dr. Michael L. Norton or refer to the Contact page for further information.