How increasing manganese concentrations affect the
metabolic processes of Chlorella
The goal of this experiment is to determine the maximum amount of
manganese that can be absorbed by Chlorella vulgaris, a
unicellular green alga, and the extent of which manganese toxicity
affects its metabolic processes.
The alga will be exposed to increasing concentrations of
manganese that has been added to the growth media.
An assay that detects manganese will then be conducted after
the cells have been lysed to ascertain the amount of manganese in the
cells. In addition to monitoring manganese levels, the concentrations
of chlorophyll a and lipids
as well as the biomass will be recorded for the various samples.
The rate of manganese accumulation in the algae can then be
used to determine how much manganese is ingested by higher-order
organisms of the food chain.
The effects on those other organisms, specifically crayfish,
are currently being studied in another research lab.