Associate Professor and Director of Graduate Studies, Biomedical Sciences Graduate Program
Department: Pharmacology, Physiology and Toxicology
Research Clusters: Cardiovascular Disease, Obesity, and Diabetes; Neuroscience and Developmental Biology
Office: BBSC 301-C
Phone: (304) 696-3531 | Fax: (304) 696-7272
The role of histamine in melanoma (collaboration with Dr. Carl A. Gruetter)
Up-regulation of histamine content and expression/activity of the histamine-forming enzyme, histidine decarboxylase (HDC) has been well documented in numerous cancer tissues/cells, including melanoma. However, the underlying mechanisms and roles of the endogenous histaminergic system in cancer progression remain unclear. This project will investigate the potential intracellular action of endogenous histamine and/or its imidazole metabolites to promote cancer cell invasiveness. Immunocytochemistry, Western blot and RT-PCR will be used to establish/verify the presence of HDC and histamine in selected melanoma and breast cancer cell lines. The role of endogenous histamine and/or its metabolites on invasiveness will be evaluated by measuring the effects of HDC blockade on invasiveness of cultured cells. Involvement of the NO/cGMP signaling pathway in histamine-related modulation of invasiveness will be evaluated and the potential synergistic interaction between hypoxia and the endogenous histaminergic system to promote invasiveness will be assessed. It is anticipated that the results from this investigation will establish an important role for the endogenous histaminergic system to promote invasiveness in melanoma.
Davis, S.C., S. Clark, J.R. Hayes, T. Green, and C.A. Gruetter. (2011) Up-regulation of histidine decarboxylase expression and histamine content in B16F10 murine melanoma cells. Inflamm Res. 60: 55-61.
Kim, E., L.M. Grover, D. Bertolotti, and T.L. Green (2010) Growth hormone rescues hippocampal synaptic function after sleep deprivation. Am. J. Phys. Regul. Integr. Comp Physiol. 298: R1588-R1596.
Tippens, A.S., S.V. Davis, J.R. Hayes, E.C. Bryda, T.L. Green and C.A. Gruetter (2004) Detection of histidine decarboxylase in rat aorta and cultured rat aortic smooth muscle cells. Inflamm. Res. 53: 390-395.
Wright, G., V. Reichenbecher, T. Green, G.L. Wright, and S. Wang (1997) Paraquat inhibits the processing of human manganese-dependent superoxide dismutase by SF-9 insect cell mitochondria. Exp. Cell Res. 234: 78-84.
Green, T.L., D.D. Hunter, W. Chan, J.P. Merlie, and J.R. Sanes (1992) Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells. J. Biol. Chem. 267: 2014-2022.