{"id":1285,"date":"2011-04-28T10:46:32","date_gmt":"2011-04-28T14:46:32","guid":{"rendered":"http:\/\/www.marshall.edu\/forensics\/?p=1285"},"modified":"2015-12-08T09:50:27","modified_gmt":"2015-12-08T14:50:27","slug":"kramer-research-poster","status":"publish","type":"post","link":"https:\/\/www.marshall.edu\/forensics\/news\/kramer-research-poster","title":{"rendered":"Research Day 2011 – Kevin Kramer – Comparison of Room Temperature Forensic DNA Extract Sample Preservation Methods"},"content":{"rendered":"

Comparison of Room Temperature Forensic DNA Extract Sample Preservation Methods<\/strong><\/strong>\u00a0<\/strong><\/p>\n

Biography:<\/strong>\u00a0<\/p>\n

Kevin Kramer is from Delphos, OH.\u00a0 He graduated from The University of Findlay in 2008 where he received a Bachelor\u2019s degree in Forensic Science with an emphasis in DNA and Serology and minors in Biology and Chemistry.\u00a0 Kevin is currently a graduate student at Marshall University where he is studying Forensic Science with emphases in DNA analysis, forensic chemistry, computer forensics, and crime scene investigation. \u00a0\u00a0This summer, Kevin his completed internship at the Marshall University Forensic Science Center evaluating room temperature storage methods of DNA extracts for the West Virginia State Police.\u00a0<\/p>\n

Abstract:<\/strong><\/p>\n

In most cases, only a portion of the entire DNA extract volume is consumed during forensic analysis.\u00a0 Once extracted, the remaining DNA is typically stored in a refrigerator at 4\u00baC, a freezer at -20\u00baC or at -70\u00baC for long-term storage to avoid sample degradation. While these are acceptable DNA storage methods, use of refrigerators and freezers may be viewed as costly when factoring in the individual cost to purchase and maintain as well as energy and space requirements. The potential loss or degradation of evidentiary samples when such systems fail must also be taken into consideration as well as when refrigeration and\/or freezers are not readily available. \u00a0For these reasons, alternative room temperature biological evidence storage systems and methods are of interest to most forensic DNA units.\u00a0 This study evaluated three room temperature storage techniques which included; Whatman\u00ae Micro FTA cards, QIAsafe\u2122 DNA Tubes, and sterile swabs.\u00a0 Swab samples were dried using the SafeSwab\u2122 swab dryer, and a contamination study was conducted to ensure that the drying process would not cause cross contamination.\u00a0 All samples were extracted with Promega\u2019s DNA IQ\u2122 system on the BIOMEK\u00ae 3000 Laboratory Automation Workstation, quantified with Applied Biosystems Quantifiler Duo\u00ae Quantification Kit on Applied Biosystems 7500 Sequence Detection System, and amplified using Promega\u2019s multiplex STR PowerPlex\u00ae <\/sup>16 system and capillary electrophoresis run on ABI Prism\u00ae 3130xl<\/em> Genetic Analyzer.\u00a0 Raw data from the 3130xl<\/em> was analyzed using Genemapper\u00ae ID v3.2.1.<\/strong><\/p>\n

Poster:<\/strong><\/p>\n

\u00a0\"Comparison<\/a><\/p>\n

References:<\/strong><\/p>\n

    \n
  1. Applied Biosystems Genemapper\u00ae\u00a0 ID\u00a0 Software v3.2.1-Human Identification Analysis, User Guide, \u00a9 Copyright 2003 Applied Biosystems.<\/li>\n
  2. Applied Biosystems, Quantifiler\u00ae Duo DNA Quantification Kit-User\u2019s Manual, Part Number 4391294 \u00a9 Copyright 2008.<\/li>\n
  3. Crowe, J. et al.<\/em>\u00a0 The Role of Vitrification in Anhydrobiosis.\u00a0 Ann. Rev. of Phys.\u00a0 1998 Oct;60:73-103.<\/li>\n
  4. GE\u00ae Healthcare, FTA\u00ae Technology. FTA\u2122 Cards Data File 51613.\u00a0 Whatman\u00ae.\u00a0 Accessed May 20, 2010 from http:\/\/www.whatman.com\/References\/FTA%20CardsDataSheet%20FINAL%203.25.10%20LR.pdf.<\/li>\n
  5. Promega\u2019s DNA IQ\u2122 System-Database Protocol PN TB297, Revised May 2009.<\/li>\n
  6. Promega\u2019s PowerPlex\u2122 16 System Technical Manual No. D012. Promega Corporation, Madison, WI.<\/li>\n
  7. Promega\u2019s Tissue and Hair Extraction Kit (for use with DNA IQ\u2122) Protocol PN TB 307, revised May 2009.<\/li>\n
  8. QIAsafe\u2122 DNA Tube and 96-Well Plate Handbook.\u00a0 Qiagen\u00ae, January 2008.<\/li>\n
  9. Smith, S., et al.<\/em> \u00a0Optional Storage Conditions for Highly Dilute DNA Samples: A Role for Trehalose as a Preserving Agent.\u00a0 J Forensic Sci. 2005 Sept;50(5):1101-1108.<\/li>\n
  10. Whatman\u00ae Storage of Purified DNA on FTA\u00ae Technology.\u00a0 Protocol for Purified Genomic DNA on FTA\u00ae.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    Comparison of Room Temperature Forensic DNA Extract Sample Preservation Methods\u00a0 Biography:\u00a0 Kevin Kramer is from Delphos, OH.\u00a0 He graduated from The University of Findlay in 2008 where he received a Bachelor\u2019s degree in Forensic Science with an emphasis in DNA and Serology and minors in Biology and Chemistry.\u00a0 Kevin is currently a graduate student at<\/p>\n","protected":false},"author":120,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[4,5],"tags":[150,113,120],"class_list":["post-1285","post","type-post","status-publish","format-standard","hentry","category-news","category-research","tag-research","tag-research-day-2011","tag-students"],"acf":[],"fimg_url":false,"_links":{"self":[{"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/posts\/1285","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/users\/120"}],"replies":[{"embeddable":true,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/comments?post=1285"}],"version-history":[{"count":0,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/posts\/1285\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/media?parent=1285"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/categories?post=1285"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/tags?post=1285"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}