{"id":1317,"date":"2011-04-28T12:02:45","date_gmt":"2011-04-28T16:02:45","guid":{"rendered":"http:\/\/www.marshall.edu\/forensics\/?p=1317"},"modified":"2015-12-08T09:50:29","modified_gmt":"2015-12-08T14:50:29","slug":"rommel-research-poster","status":"publish","type":"post","link":"https:\/\/www.marshall.edu\/forensics\/news\/rommel-research-poster","title":{"rendered":"Research Day 2011 – Megan Rommel – Comparative Evaluation of Manual Extraction Methods for the Biology\/DNA Detail of the Las Vegas Metropolitan Police Department Forensic Laboratory"},"content":{"rendered":"

Comparative Evaluation of Manual Extraction Methods for the Biology\/DNA Detail of the Las Vegas Metropolitan Police Department Forensic Laboratory<\/strong><\/p>\n

Biography:<\/strong><\/p>\n

Megan Rommel is from Dallas, Texas.\u00a0 She graduated from Duke University in 2009 with a Bachelor of Science degree in Biology, concentrating in Genetics, and minors in Chemistry and Psychology.\u00a0 She is a second year graduate student in the Master\u2019s of Forensic Science Program at Marshall University, completing areas of emphasis in forensic DNA analysis, crime scene investigation, and computer forensics.\u00a0 Megan completed her summer internship with the Las Vegas Metropolitan Police Department in the Forensic Laboratory\u2019s Biology\/DNA Detail.<\/p>\n

Abstract:\u00a0<\/strong><\/p>\n

An extraction method for forensic DNA casework must produce a high quantitative yield as well as a robust STR profile free from artifacts across all sample types, including low level samples such as touch DNA.\u00a0 The Las Vegas Metropolitan Police Department Forensic Laboratory Biology\/DNA Detail has previously relied on phenol-chloroform organic extraction for forensic casework.\u00a0 In order to expedite the extraction process and foray into automation instruments, a comparison study was undertaken with Applied Biosystems\u2019 PrepFilerTM<\/sup> Forensic DNA Extraction Kit and Qiagen\u2019s QIAamp\u00ae DNA Investigator Kit for the purpose of determining a manual extraction chemistry to replace organic extractions and to be automated in the future.\u00a0 The kits were evaluated on the basis of I) contamination issues II) quantitative yield III) STR profile quality IV) future automation potential V) time consumption of the method and VI) cost of the method.\u00a0 Based on the results of this study, LVMPD chose to validate the PrepFilerTM<\/sup> Forensic DNA Extraction Kit, citing higher quantitative yields for low level samples and better detection of minor contributors in mixture samples than the other methods, along with STR profile quality comparable to an organic extraction.<\/p>\n

Poster:<\/strong><\/p>\n

\"Comparative<\/a><\/strong><\/p>\n

References:<\/strong><\/p>\n

    \n
  1. Applied Biosystems.\u00a0 AmpFl<\/em>STR\u00ae Identifiler\u00ae Plus PCR Amplification Kit User\u2019s Guide.\u00a0 Foster City, CA: Applied Biosystems, 2009; Part #4402743 Rev. A.<\/li>\n
  2. Applied Biosystems.\u00a0 PrepFilerTM<\/sup> Forensic DNA Extraction Kit User Guide.\u00a0 Foster City, CA: Applied Biosystems, 2008; Part #4390932 Rev. B.<\/li>\n
  3. Barbaro, A, Cormaci P, Agostino A.\u00a0 Validation of PrepFilerTM<\/sup> forensic DNA extraction kit (Applied Biosystems).\u00a0 Forensic Sci Int 2009; 2: 176-177.<\/li>\n
  4. Brevnov MG, Pawar HS, Mundt J, Calandro LM, Furtado MR, Shewale JG.\u00a0 Developmental Validation of the PrepFilerTM <\/sup>Forensic DNA Extraction Kit for Extraction of Genomic DNA from Biological Samples. J Forensic Sci 2009 May; 54(3): 599-607.<\/li>\n
  5. Las Vegas Metropolitan Police Department Forensic Laboratory.\u00a0 DNA Procedure Manual.\u00a0 Las Vegas: LVMPD, 2009.<\/li>\n
  6. Promega Corporation.\u00a0 Plexor\u00ae HY System for the Applied Biosystems 7500 and 7500 FAST Real-Time PCR Systems Technical Manual. Madison, WI: Promega Corporation, 2007; Part #TM293. Rev A.<\/li>\n
  7. Qiagen Incorporated.\u00a0 QIAamp\u00ae DNA Investigator Handbook.\u00a0 Valencia, CA: Qiagen Incorporated, 2007; Part #56504.<\/li>\n
  8. Wickenheiser RA.\u00a0 Trace DNA: A Review, Discussion of Theory, and Application of the Transfer of Trace Quantities of DNA Through Skin Contact.\u00a0 J Forensic Sci 2002; 47(3): 442-450<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    Comparative Evaluation of Manual Extraction Methods for the Biology\/DNA Detail of the Las Vegas Metropolitan Police Department Forensic Laboratory Biography: Megan Rommel is from Dallas, Texas.\u00a0 She graduated from Duke University in 2009 with a Bachelor of Science degree in Biology, concentrating in Genetics, and minors in Chemistry and Psychology.\u00a0 She is a second year<\/p>\n","protected":false},"author":120,"featured_media":1319,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[4,5],"tags":[150,113,120],"class_list":["post-1317","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news","category-research","tag-research","tag-research-day-2011","tag-students"],"acf":[],"fimg_url":"https:\/\/www.marshall.edu\/forensics\/files\/2011\/04\/rommel.jpg","_links":{"self":[{"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/posts\/1317","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/users\/120"}],"replies":[{"embeddable":true,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/comments?post=1317"}],"version-history":[{"count":0,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/posts\/1317\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/media\/1319"}],"wp:attachment":[{"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/media?parent=1317"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/categories?post=1317"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.marshall.edu\/forensics\/wp-json\/wp\/v2\/tags?post=1317"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}