Sandrine Pierre, Ph.D.

Sandrine Pierre, Ph.D.

Exploring new regulatory mechanisms in cardiovascular and renal physiology

How do membrane Na+/K+-ATPases regulate cellular function and change with disease?

The integrity of Na+/K+-ATPase protein complexes at the cell membrane is critical for the maintenance of ion homeostasis. It is also key to adequate initiation and transduction of signaling by cardiotonic steroids and subsequent developmental, physiological, and pharmacological effects.

We utilize genetic approaches and preclinical animal models, as well as cultured cell lines to understand the molecular mechanisms of Na+/K+-ATPase receptor function and develop novel approaches for therapeutic interventions in major renal and cardiovascular diseases such as hypertension, myocardial infarction, and heart failure.

Professional Appointments

Selected Publications

For a more complete list of publications, visit the following publication sites: PubMed (

Pierre SV, Blanco G. Na/K-ATPase Ion Transport and Receptor-Mediated Signaling Pathways. J Membr Biol. 2021 Dec;254(5-6):443-446. doi: 10.1007/s00232-021-00207-9. Epub 2021 Nov 1. PubMed PMID: 34724099.

Maixent JM, Pierre SV, Sadrin S, Guieu R, Paganelli F. Effects of long-term anti-ischemic drug treatment on Na,K-ATPase isoforms in cardiomyopathic hamsters. Cell Mol Biol (Noisy-le-grand). 2021 Aug 31;67(2):76-82. doi: 10.14715/cmb/2021.67.2.11. PubMed PMID: 34817336.

Kutz LC, Cui X, Xie JX, Mukherji ST, Terrell KC, Huang M, Wang X, Wang J, Martin AJ, Pessoa MT, Cai L, Zhu H, Heiny JA, Shapiro JI, Blanco G, Xie Z, Pierre SV. The Na/K-ATPase α1/Src interaction regulates metabolic reserve and Western diet intolerance. Acta Physiol (Oxf). 2021 Jul;232(3):e13652. doi: 10.1111/apha.13652. Epub 2021 Apr 4. PubMed PMID: 33752256; PubMed Central PMCID: PMC8570534.

Banerjee M, Li Z, Gao Y, Lai F, Huang M, Zhang Z, Cai L, Sanabria J, Gao T, Xie Z, Pierre SV. Inverse agonism at the Na/K-ATPase receptor reverses EMT in prostate cancer cells. Prostate. 2021 Jul;81(10):667-682. doi: 10.1002/pros.24144. Epub 2021 May 6. PubMed PMID: 33956349.

Marck PV, Pessoa MT, Xu Y, Kutz LC, Collins DM, Yan Y, King C, Wang X, Duan Q, Cai L, Xie JX, Lingrel JB, Xie Z, Tian J, Pierre SV. Cardiac Oxidative Signaling and Physiological Hypertrophy in the Na/K-ATPase α1s/sα2s/s Mouse Model of High Affinity for Cardiotonic Steroids. Int J Mol Sci. 2021 Mar 27;22(7). doi: 10.3390/ijms22073462. PubMed PMID: 33801629; PubMed Central PMCID: PMC8036649.

Wang J, Wang X, Gao Y, Lin Z, Chen J, Gigantelli J, Shapiro JI, Xie Z, Pierre SV. Stress Signal Regulation by Na/K-ATPase As a New Approach to Promote Physiological Revascularization in a Mouse Model of Ischemic Retinopathy. Invest Ophthalmol Vis Sci. 2020 Dec 1;61(14):9. doi: 10.1167/iovs.61.14.9. PubMed PMID: 33275652; PubMed Central PMCID: PMC7718810.

Wang X, Cai L, Xie JX, Cui X, Zhang J, Wang J, Chen Y, Larre I, Shapiro JI, Pierre SV, Wu D, Zhu GZ, Xie Z. A caveolin binding motif in Na/K-ATPase is required for stem cell differentiation and organogenesis in mammals and C.elegans. Sci Adv. 2020 May;6(22):eaaw5851. doi: 10.1126/sciadv.aaw5851. eCollection 2020 May. PubMed PMID: 32537485; PubMed Central PMCID: PMC7253156.

Qi Y, Yang C, Jiang Z, Wang Y, Zhu F, Li T, Wan X, Xu Y, Xie Z, Li D, Pierre SV. Epicatechin-3-Gallate Signaling and Protection against Cardiac Ischemia/Reperfusion Injury. J Pharmacol Exp Ther. 2019 Dec;371(3):663-674. doi: 10.1124/jpet.119.260117. Epub 2019 Oct 3. PubMed PMID: 31582423.

Buzaglo N, Golomb M, Rosen H, Beeri R, Ami HC, Langane F, Pierre S, Lichtstein D. Augmentation of Ouabain-Induced Increase in Heart Muscle Contractility by Akt Inhibitor MK-2206. J Cardiovasc Pharmacol Ther. 2019 Jan;24(1):78-89. doi: 10.1177/1074248418788301. Epub 2018 Jul 22. PubMed PMID: 30033751.

Marck PV, Pierre SV. Na/K-ATPase Signaling and Cardiac Pre/Postconditioning with Cardiotonic Steroids. Int J Mol Sci. 2018 Aug 9;19(8). doi: 10.3390/ijms19082336. Review. PubMed PMID: 30096873; PubMed Central PMCID: PMC6121447.

Kutz LC, Mukherji ST, Wang X, Bryant A, Larre I, Heiny JA, Lingrel JB, Pierre SV, Xie Z. Isoform-specific role of Na/K-ATPase α1 in skeletal muscle. Am J Physiol Endocrinol Metab. 2018 Jun 1;314(6):E620-E629. doi: 10.1152/ajpendo.00275.2017. Epub 2018 Feb 13. PubMed PMID: 29438630; PubMed Central PMCID: PMC6032065.

Yu H, Cui X, Zhang J, Xie JX, Banerjee M, Pierre SV, Xie Z. Heterogeneity of signal transduction by Na-K-ATPase α-isoforms: role of Src interaction. Am J Physiol Cell Physiol. 2018 Feb 1;314(2):C202-C210. doi: 10.1152/ajpcell.00124.2017. Epub 2017 Nov 8. PubMed PMID: 29118027; PubMed Central PMCID: PMC5866435.

Duan Q, Xu Y, Marck PV, Kalisz J, Morgan EE, Pierre SV. Preconditioning and Postconditioning by Cardiac Glycosides in the Mouse Heart. J Cardiovasc Pharmacol. 2018 Feb;71(2):95-103. doi: 10.1097/FJC.0000000000000549. PubMed PMID: 29419572; NIHMSID:NIHMS912903.

Madan N, Xu Y, Duan Q, Banerjee M, Larre I, Pierre SV, Xie Z. Src-independent ERK signaling through the rat α3 isoform of Na/K-ATPase. Am J Physiol Cell Physiol. 2017 Mar 1;312(3):C222-C232. doi: 10.1152/ajpcell.00199.2016. Epub 2016 Nov 30. PubMed PMID: 27903584; PubMed Central PMCID: PMC5401946.

Belliard A, Gulati GK, Duan Q, Alves R, Brewer S, Madan N, Sottejeau Y, Wang X, Kalisz J, Pierre SV. Ischemia/reperfusion-induced alterations of enzymatic and signaling functions of the rat cardiac Na+/K+-ATPase: protection by ouabain preconditioning. Physiol Rep. 2016 Oct;4(19). doi: 10.14814/phy2.12991. PubMed PMID: 27702882; PubMed Central PMCID: PMC5064143.

Xie J, Ye Q, Cui X, Madan N, Yi Q, Pierre SV, Xie Z. Expression of rat Na-K-ATPase α2 enables ion pumping but not ouabain-induced signaling in α1-deficient porcine renal epithelial cells. Am J Physiol Cell Physiol. 2015 Sep 15;309(6):C373-82. doi: 10.1152/ajpcell.00103.2015. Epub 2015 Jun 24. PubMed PMID: 26108663; PubMed Central PMCID: PMC4572368.

Duan Q, Madan ND, Wu J, Kalisz J, Doshi KY, Haldar SM, Liu L, Pierre SV. Role of phosphoinositide 3-kinase IA (PI3K-IA) activation in cardioprotection induced by ouabain preconditioning. J Mol Cell Cardiol. 2015 Mar;80:114-25. doi: 10.1016/j.yjmcc.2014.12.021. Epub 2015 Jan 7. PubMed PMID: 25575882; PubMed Central PMCID: PMC4827870.

Najjar SM, Ledford KJ, Abdallah SL, Paus A, Russo L, Kaw MK, Ramakrishnan SK, Muturi HT, Raphael CK, Lester SG, Heinrich G, Pierre SV, Benndorf R, Kleff V, Jaffa AA, Lévy E, Vazquez G, Goldberg IJ, Beauchemin N, Scalia R, Ergün S. Ceacam1 deletion causes vascular alterations in large vessels. Am J Physiol Endocrinol Metab. 2013 Aug 15;305(4):E519-29. doi: 10.1152/ajpendo.00266.2013. Epub 2013 Jun 25. PubMed PMID: 23800882; PubMed Central PMCID: PMC3891225.

Bai Y, Morgan EE, Giovannucci DR, Pierre SV, Philipson KD, Askari A, Liu L. Different roles of the cardiac Na+/Ca2+-exchanger in ouabain-induced inotropy, cell signaling, and hypertrophy. Am J Physiol Heart Circ Physiol. 2013 Feb 1;304(3):H427-35. doi: 10.1152/ajpheart.00462.2012. Epub 2012 Nov 30. PubMed PMID: 23203972; PubMed Central PMCID: PMC3774505.

Belliard A, Sottejeau Y, Duan Q, Karabin JL, Pierre SV. Modulation of cardiac Na+,K+-ATPase cell surface abundance by simulated ischemia-reperfusion and ouabain preconditioning. Am J Physiol Heart Circ Physiol. 2013 Jan 1;304(1):H94-103. doi: 10.1152/ajpheart.00374.2012. Epub 2012 Oct 19. PubMed PMID: 23086991; PubMed Central PMCID: PMC3543687.

Pierre SV, Belliard A, Sottejeau Y. Modulation of Na(+)-K(+)-ATPase cell surface abundance through structural determinants on the α1-subunit. Am J Physiol Cell Physiol. 2011 Jan;300(1):C42-8. doi: 10.1152/ajpcell.00386.2010. Epub 2010 Nov 3. PubMed PMID: 21048163; PubMed Central PMCID: PMC3023198.

Duran MJ, Pierre SV, Lesnik P, Pieroni G, Bourdeaux M, Dignat-Georges F, Sampol J, Maixent JM. 7-ketocholesterol inhibits Na,K-ATPase activity by decreasing expression of its α1-subunit and membrane fluidity in human endothelial cells. Cell Mol Biol (Noisy-le-grand). 2010 Nov 9;56 Suppl:OL1434-41. PubMed PMID: 21062577.

Sottejeau Y, Patel AM, Gerber G, Pierre SV, Maixent JM. Effect of a novel Omegacoeur®/Doluperine® nutritional combination on human embryonic kidney cell viability. Cell Mol Biol (Noisy-le-grand). 2010 Oct 5;56 Suppl:OL1400-9. PubMed PMID: 21062574.

Quintas LE, Pierre SV, Liu L, Bai Y, Liu X, Xie ZJ. Alterations of Na+/K+-ATPase function in caveolin-1 knockout cardiac fibroblasts. J Mol Cell Cardiol. 2010 Sep;49(3):525-31. doi: 10.1016/j.yjmcc.2010.04.015. Epub 2010 May 6. PubMed PMID: 20451529; PubMed Central PMCID: PMC5375041.

Sottejeau Y, Belliard A, Duran MJ, Pressley TA, Pierre SV. Critical role of the isoform-specific region in alpha1-Na,K-ATPase trafficking and protein Kinase C-dependent regulation. Biochemistry. 2010 May 4;49(17):3602-10. doi: 10.1021/bi9021999. PubMed PMID: 20302352; PubMed Central PMCID: PMC4303032.

Morgan EE, Li Z, Stebal C, Belliard A, Tennyson G, Salari B, Garlid KD, Pierre SV. Preconditioning by subinotropic doses of ouabain in the Langendorff perfused rabbit heart. J Cardiovasc Pharmacol. 2010 Mar;55(3):234-9. doi: 10.1097/FJC.0b013e3181ce5e14. PubMed PMID: 20010435; PubMed Central PMCID: PMC4303028.

Garlid KD, Costa AD, Quinlan CL, Pierre SV, Dos Santos P. Cardioprotective signaling to mitochondria. J Mol Cell Cardiol. 2009 Jun;46(6):858-66. doi: 10.1016/j.yjmcc.2008.11.019. Epub 2008 Dec 11. Review. PubMed PMID: 19118560; PubMed Central PMCID: PMC2683183.

Quinlan CL, Costa AD, Costa CL, Pierre SV, Dos Santos P, Garlid KD. Conditioning the heart induces formation of signalosomes that interact with mitochondria to open mitoKATP channels. Am J Physiol Heart Circ Physiol. 2008 Sep;295(3):H953-H961. doi: 10.1152/ajpheart.00520.2008. Epub 2008 Jul 11. PubMed PMID: 18621853; PubMed Central PMCID: PMC2544503.

Pierre SV, Sottejeau Y, Gourbeau JM, Sánchez G, Shidyak A, Blanco G. Isoform specificity of Na-K-ATPase-mediated ouabain signaling. Am J Physiol Renal Physiol. 2008 Apr;294(4):F859-66. doi: 10.1152/ajprenal.00089.2007. Epub 2007 Dec 19. PubMed PMID: 18094034.

Costa AD, Pierre SV, Cohen MV, Downey JM, Garlid KD. cGMP signalling in pre- and post-conditioning: the role of mitochondria. Cardiovasc Res. 2008 Jan 15;77(2):344-52. doi: 10.1093/cvr/cvm050. Epub 2007 Oct 25. Review. PubMed PMID: 18006449.

Liu L, Zhao X, Pierre SV, Askari A. Association of PI3K-Akt signaling pathway with digitalis-induced hypertrophy of cardiac myocytes. Am J Physiol Cell Physiol. 2007 Nov;293(5):C1489-97. doi: 10.1152/ajpcell.00158.2007. Epub 2007 Aug 29. PubMed PMID: 17728397.

Bonello L, Sbragia P, Amabile N, Com O, Pierre SV, Levy S, Paganelli F. Protective effect of an acute oral loading dose of trimetazidine on myocardial injury following percutaneous coronary intervention. Heart. 2007 Jun;93(6):703-7. doi: 10.1136/hrt.2006.107524. Epub 2007 May 8. PubMed PMID: 17488771; PubMed Central PMCID: PMC1955183.

Liang M, Tian J, Liu L, Pierre S, Liu J, Shapiro J, Xie ZJ. Identification of a pool of non-pumping Na/K-ATPase. J Biol Chem. 2007 Apr 6;282(14):10585-93. doi: 10.1074/jbc.M609181200. Epub 2007 Feb 12. PubMed PMID: 17296611.

Pasdois P, Quinlan CL, Rissa A, Tariosse L, Vinassa B, Costa AD, Pierre SV, Dos Santos P, Garlid KD. Ouabain protects rat hearts against ischemia-reperfusion injury via pathway involving src kinase, mitoKATP, and ROS. Am J Physiol Heart Circ Physiol. 2007 Mar;292(3):H1470-8. doi: 10.1152/ajpheart.00877.2006. Epub 2006 Nov 10. PubMed PMID: 17098831.

Pierre SV, Yang C, Yuan Z, Seminerio J, Mouas C, Garlid KD, Dos-Santos P, Xie Z. Ouabain triggers preconditioning through activation of the Na+,K+-ATPase signaling cascade in rat hearts. Cardiovasc Res. 2007 Feb 1;73(3):488-96. doi: 10.1016/j.cardiores.2006.11.003. Epub 2006 Nov 6. PubMed PMID: 17157283; PubMed Central PMCID: PMC1852501.

Liu L, Li J, Liu J, Yuan Z, Pierre SV, Qu W, Zhao X, Xie Z. Involvement of Na+/K+-ATPase in hydrogen peroxide-induced hypertrophy in cardiac myocytes. Free Radic Biol Med. 2006 Nov 15;41(10):1548-56. doi: 10.1016/j.freeradbiomed.2006.08.018. Epub 2006 Aug 25. PubMed PMID: 17045923.

Pierre SV, Xie Z. The Na,K-ATPase receptor complex: its organization and membership. Cell Biochem Biophys. 2006;46(3):303-16. doi: 10.1385/cbb:46:3:303. Review. PubMed PMID: 17272855.

Duran MJ, Pierre SV, Carr DL, Pressley TA. The isoform-specific region of the Na,K-ATPase catalytic subunit: role in enzyme kinetics and regulation by protein kinase C. Biochemistry. 2004 Dec 28;43(51):16174-83. doi: 10.1021/bi0490183. PubMed PMID: 15610011.

Pierre SV, Duran MJ, Carr DL, Pressley TA. Structure/function analysis of Na(+)-K(+)-ATPase central isoform-specific region: involvement in PKC regulation. Am J Physiol Renal Physiol. 2002 Nov;283(5):F1066-74. doi: 10.1152/ajprenal.00153.2002. PubMed PMID: 12372782.

Maixent JM, Duran MJ, Pierre S, Sennoune S, Robert K, Bernard M, Lévy S. Remodeling of Na,K-ATPase, and membrane fluidity after atrial fibrillation in sheep. J Recept Signal Transduct Res. 2002 Feb-Nov;22(1-4):201-11. doi: 10.1081/rrs-120014596. PubMed PMID: 12503616.

Pierre S, Paganelli F, Sennoune S, Roche R, Schwinger RH, Mesana T, Maixent JM. RT-PCR detection of the Na,K-ATPase beta3-isoform in human heart. Cell Mol Biol (Noisy-le-grand). 2001 Mar;47(2):261-4. PubMed PMID: 11354999.

Pierre S, Compe E, Grillasca JP, Plannells R, Sampol J, Pressley TA, Maixent JM. RT-PCR detection of Na,K-ATPase subunit isoforms in human umbilical vein endothelial cells (HUVEC): evidence for the presence of alpha1 and beta3. Cell Mol Biol (Noisy-le-grand). 2001 Mar;47(2):319-24. PubMed PMID: 11355007.

Maixent JM, Barbey O, Pierre S, Duran MJ, Sennoune S, Bourdeaux M, Ricard P, Lévy S. Inhibition of Na,K-ATPase by external electrical cardioversion in a sheep model of atrial fibrillation. J Cardiovasc Electrophysiol. 2000 Apr;11(4):439-45. doi: 10.1111/j.1540-8167.2000.tb00340.x. PubMed PMID: 10809498.

Barbey O, Pierre S, Duran MJ, Sennoune S, Lévy S, Maixent JM. Specific up-regulation of mitochondrial F0F1-ATPase activity after short episodes of atrial fibrillation in sheep. J Cardiovasc Electrophysiol. 2000 Apr;11(4):432-8. doi: 10.1111/j.1540-8167.2000.tb00339.x. PubMed PMID: 10809497.

Sennoune S, Gerbi A, Duran MJ, Grillasca JP, Compe E, Pierre S, Planells R, Bourdeaux M, Vague P, Pieroni G, Maixent JM. Effect of streptozotocin-induced diabetes on rat liver Na+/K+-ATPase. Eur J Biochem. 2000 Apr;267(7):2071-8. doi: 10.1046/j.1432-1327.2000.01211.x. PubMed PMID: 10727947.

Gerbi A, Sennoune S, Pierre S, Sampol J, Raccah D, Vague P, Maixent JM. Localization of Na,K-ATPase alpha/beta isoforms in rat sciatic nerves: effect of diabetes and fish oil treatment. J Neurochem. 1999 Aug;73(2):719-26. doi: 10.1046/j.1471-4159.1999.0730719.x. PubMed PMID: 10428069.

Sennoune S, Gerbi A, Duran MJ, Benkoël L, Pierre S, Lambert R, Dodero F, Chamlian A, Vague P, Maixent JM. A quantitative immunocytochemical study of Na+,K+-ATPase in rat hepatocytes after STZ-induced diabetes and dietary fish oil supplementation. J Histochem Cytochem. 1999 Jun;47(6):809-16. doi: 10.1177/002215549904700610. PubMed PMID: 10330457.

Pierre S, Jamme I, Droy-Lefaix MT, Nouvelot A, Maixent JM. Ginkgo biloba extract (EGb 761) protects Na,K-ATPase activity during cerebral ischemia in mice. Neuroreport. 1999 Jan 18;10(1):47-51. doi: 10.1097/00001756-199901180-00009. PubMed PMID: 10094131.

Barbey O, Gerbi A, Robert K, Mayol V, Pierre S, Paganelli F, Maixent JM. Immunological identification of Na,K-ATPase isoforms in nonfailing and failing myocardium. Ann N Y Acad Sci. 1997 Nov 3;834:656-7. doi: 10.1111/j.1749-6632.1997.tb52342.x. PubMed PMID: 9405884.

Previous Positions

Associate Investigator & Associate Scientific Director, MIIR

Associate Professor, Department of Biochemistry & Cancer Biology, University of Toledo College of Medicine

Assistant Professor, Department of Physiology and Pharmacology, University of Toledo College of Medicine

Education and Training

Texas Tech University Health Sciences Center: Postdoctoral Fellowship

University of Aix-Marseille II: Ph.D., Cellular Endocrinology

University of Aix-Marseille II:  B.S., Cell Biology/Immunology